Journal: Journal of ethnopharmacology

Article Title: Buzhong Yiqi Decoction Improves Inflammation and Oxidative Damage in Autoimmune Thyroiditis by Inhibiting Apoptosis via the SIRT1-Mediated Nrf2/NF-κB Axis.

doi: 10.1016/j.jep.2025.119967

Figure Lengend Snippet: Fig. 4. BZYQ can regulate the expression of SIRT1, NF-κB p65, Nrf2, and HO-1 in the 402

Article Snippet: The PVDF 305 membrane was blocked in 5% skim milk for 1.5 h at the room temperature and 306 incubated overnight at 4°C with specific antibodies, including SIRT1 (1:3000, 307 ProteinTech Group, Wuhan, China), NFκB-p65 Polyclonal Antibody (1:3000dilution, 308 ProteinTech Group), NFκB-p65 (phospho Ser536) Polyclonal Antibody (1:100 dilution, 309 ImmunoWay Biotechnology Company, Texas, USA), Nrf2 Polyclonal Antibody 310 (1:1000 dilution, ImmunoWay Biotechnology Company), HO-1 Mouse mAb (Mix-311 mA) (1:1500 dilution, ImmunoWay Biotechnology Company), Bax mouse 312 Monoclonal Antibody (6F11) (1:1500 dilution, ImmunoWay Biotechnology 313 Company), Bcl-2 Cytochrome c Monoclonal antibody (1:5000 dilution, ProteinTech 314 Group), Caspase-3/p17/p19 Monoclonal antibody (1:2000 dilution, ProteinTech 315 Group), and GAPDH (1:10000, ProteinTech Group).

Techniques: Expressing

Journal: Biofabrication

Article Title: Chlorella -enriched hydrogels protect against myocardial damage and reactive oxygen species production in an in vitro ischemia/reperfusion model using cardiac spheroids.

doi: 10.1088/1758-5090/ad8266

Figure Lengend Snippet: Figure 1. Schematic representation of the experimental plan. (A) Hydrogels enriched with Chlorella vulgaris cells were prepared in sterile conditions and fully characterised by Brillouin microspectroscopy, shear rheology, unconfined compression testing, and scanning electron microscopy. (B) HCAECs, HCFs, and iCMs were first cultured separately, then co-cultured in non-adherent agarose microwells, and incubated until compact CSs were formed. The CSs were plated in 96-well plates and embedded in Alg–Gel hydrogels. (C) CSs embedded in Alg–Gel hydrogels cultured in I/R and normoxic conditions before assessing the potential impact on cardiac cell viability, as well as their ROS scavenging activity. Finally, any effects of Chlorella vulgaris on cell ratio in CSs were determined by immunostaining analyses.

Article Snippet: Briefly, human cardiac fibroblasts (HCFs) and human coronary artery endothelial cells (HCAECs) were obtained from Cell Applications (San Diego, CA, United States) and cultured in HCFs Medium and MesoEndo Growth Medium (Cell Applications, Inc, San Diego, CA, United States. iCell® Cardiomyocytes (iCMs) were obtained from FujiFilm Cellular Dynamics (Madison, WI, USA). iCMs were plated in iCell® Plating Medium for one day and then cultured in iCell®MaintenanceMedium for 2 d (Fujifilm Cellular Dynamics, Inc., Madison, Wisconsin, USA) according to the manufacturer protocol.

Techniques: Sterility, Shear, Electron Microscopy, Cell Culture, Incubation, Activity Assay, Immunostaining

Journal: Biofabrication

Article Title: Chlorella -enriched hydrogels protect against myocardial damage and reactive oxygen species production in an in vitro ischemia/reperfusion model using cardiac spheroids.

doi: 10.1088/1758-5090/ad8266

Figure Lengend Snippet: Figure 8. The addition of algae does not alter the presence of cardiac endothelial, myocytes and fibroblasts after 28 d in co-culture. Collapsed z-stacks of confocal images of representative CSs after 28 d of incubation with AlgGel and Chlorella-enriched hydrogel. (A) The CSs were stained with antibodies against Vimentin (green) for HCFs, cTNT (red) for iCMs, and CD31 (blue) for HCAECs. (B) Statistical analyses of stained cells fluorescence using Brown–Forsythe and Welch ANOVA test to compare the groups. Error bars represent the mean ± SEM, if not visible they are within the symbols (n ⩾3). Scale bars in images are equal to 100 µm.

Article Snippet: Briefly, human cardiac fibroblasts (HCFs) and human coronary artery endothelial cells (HCAECs) were obtained from Cell Applications (San Diego, CA, United States) and cultured in HCFs Medium and MesoEndo Growth Medium (Cell Applications, Inc, San Diego, CA, United States. iCell® Cardiomyocytes (iCMs) were obtained from FujiFilm Cellular Dynamics (Madison, WI, USA). iCMs were plated in iCell® Plating Medium for one day and then cultured in iCell®MaintenanceMedium for 2 d (Fujifilm Cellular Dynamics, Inc., Madison, Wisconsin, USA) according to the manufacturer protocol.

Techniques: Algae, Co-Culture Assay, Incubation, Staining, Fluorescence